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In this specimen, 125l bound to nerve growth factor (NGF) was injected into the animal, and the tissue was removed 1 hourlater.The specimen was prepared in amanner similar to thatfor light microscopy.The relativelysmall size ofthe silver grainsaids precise localization ofthe 125l—NGF complexes. Medial to theinternal iliac artery and lateral to the ureter and in the direction of the coccyx buy provigil prescription thepararectal space is developed.

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